3-22 Changing the Mode of Operation
Sprayer assembly:
12. Screw the sprayer back into the assembly.
13. Replace the sprayer cover.
14. On the MassLynx MS Tune window, ensure that the Capillary
parameter on the ES+/- Source tab is set to 0 kV.
15. Carefully push the stage back into the NanoFlow source enclosure, using
the stop and handle.
Positioning the borosilicate glass capillary tip
Having obtained a signal, you must adjust the tip position to maximize it.
Using the three-axis manipulator, you can adjust the tip position up and
down, left and right, forward and backward. As a starting point, set the tip so
that it is on the center line of the sampling cone and at a distance between two
Caution: To ensure that the capillary tip does not collide with the
cone or the side of the source, adjust the sprayer tip position before
you push the sprayer inside the NanoFlow source enclosure.
PTFE tubing
Ferrule
Union
Knurled nut
Blue conductive elastomer
Glass capillary
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