requires about 225-mL (about 1.5 bottles). Avoid over filling
and flooding the instrument.
6. Without opening the drain port, press and hold the reservoir
pump push button for approximately 30 seconds to prime the
heat transfer fluid flow through the freezing chamber.
7. Remove the funnel and close the heat transfer fluid port by
installing the vented fluid fill plug.
8. A spare supply of heat transfer fluid should be ordered immedi-
ately. Heat transfer fluid is gradually expended during operation
because a small amount clings to the outside of each sample
tube tested; also, some fluid gradually evaporates. When the
fluid level reaches the “Fluid Level= Empty” indicator, heat
transfer fluid will again be required.
The heat transfer fluid reservoir status may be checked before it
reaches “Empty” by means of the “Reservoir Status” SETUP
menu item (see section K).
H. Self-diagnostic test
During power-up, the scrolled display, “Press START to
Continue”, indicates that the instrument is required to check for
proper operation by running a test sample in a cassette. Only a sin-
gle sample will be run at this time. The instrument operator should
load and start the self-diagnostic test as follows:
1. Pipette or measure a 2-mL sample (H
2
O is OK) into a sample
tube and place it in the first position in a sample tube cassette.
2. Place the cassette in the left (input) deck (see figure 5), with
the cassette identification number facing you.
3. Press START.
11
Installation & Setup
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