266 Agilent 1260 Infinity II Preparative Autosampler User Manual
14
Appendix
Solvent Information
Solvent Information
Observe the following recommendations on the use of solvents.
• Brown glass ware can avoid growth of algae.
• Avoid the use of the following steel-corrosive solvents:
• solutions of alkali halides and their respective acids (for example,
lithium iodide, potassium chloride, and so on),
• high concentrations of inorganic acids like sulfuric acid and nitric acid,
especially at higher temperatures (if your chromatography method
allows, replace by phosphoric acid or phosphate buffer which are less
corrosive against stainless steel),
• halogenated solvents or mixtures which form radicals and/or acids, for
example:
2CHCl
3
+ O
2
→ 2COCl
2
+ 2HCl
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the
stabilizing alcohol,
• chromatographic grade ethers, which can contain peroxides (for
example, THF, dioxane, di-isopropyl ether) should be filtered through
dry aluminium oxide which adsorbs the peroxides,
• solvents containing strong complexing agents (e.g. EDTA),
• mixtures of carbon tetrachloride with 2-propanol or THF.
• Avoid the use of dimethyl formamide (DMF). Polyvinylidene fluoride
(PVDF), which is used in leak sensors, is not resistant to DMF.
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