www.amgmicro.com 10
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User Guide
REV B Published 20 SEP 2010
BASIC OPERATION
Note: When the Color option is o, overexposed
pixels will appear red. Dim the illumination
until the red highlights disappear to get the
maximum level of brightness without any
overexposed areas. See p. 27 for instructions on
changing the overexposed pixel display.
For the color camera version, the overexposed
pixels are always highlighted in red unless this
feature is disabled in the settings.
14. Click the Capture button to acquire the image.
15. Move the light cube selection lever to the next position
and repeat steps 10-14 to acquire each fluorescence
channel as desired.
16. Click the Overlay tab to show all channels in color
overlay.
17. Adjust brightness and contrast for each channel to bring
them into balance with each other.
18. Click the Save button to save the color image. The
Save File dialog box will pop up.
19. Click in the Save File Name text field to enter the file name.
A virtual keyboard will pop up. After entering the file name,
click the Accept button at the lower right of the keyboard.
20. Choose the file type and click the Save button .
Note: See Saving Images & Working with Files (p.14)
for more information.
HELPFUL TIPS
The Boost option
10
amplifies faint signals near the
background. This only changes the display; it does not
alter the data.
Turn o the Color option to display the image in grayscale.
This often shows more detail than a color image.
Find & Focus uses a shorter exposure time (100 ms)
and lower illumination (appx. 60%) compared to image
capture settings. This minimizes photobleaching and
phototoxicity eects. When you capture an image,
illumination and exposure time automatically adjust for
best image quality and then reset to lower levels after
the capture.
The Actual tab provides full-powered illumination and actual
exposure times for live viewing of the sample.
Note: With longer exposure times (more than 200ms)
there will be a lag between focusing the image
and seeing the focus change onscreen.
Actual tab
Illumination slider and Capture button in the control bar
1 3
Color and Boost
10
options; Save button
10
5
2
Overlay tab
4
Virtual keyboard
7
Save File dialog box
6
8 9
Fluorescence Operation, continued
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