Function and setup
qTOWER
3
/ qTOWER
3
G Edition 03.19 21
5 Function and setup
The qTOWER³ combines a PCR thermocycler with a patented fluorescence photome-
ter.
1 upper part with fluores-
cence photometer
2 heated lid
3 Sample block
4 thermal cycler
Fig. 1 Layout of the qTOWER³
5.1 Fluorescence spectrometer
An 8-channel Epi fluorescence photometer with fiber -multiplexer and mechanical scan-
ning device is used as detector.
Light for exciting the dyes is emitted by four long-life LEDs in blue, green, white and red.
The light is passed through optic fibers to collimator lenses, bundled and then trans-
ferred to the excitation filter of the color modules fitted to a rotating filter wheel. The light
is deflected via a beam divider and passed through additional optic fibers to a lens array
in a shuttle system scanning the sample block in columns. The light excites the fluores-
cence dyes in the reaction mix. The fluorescence dyes then emit a light of longer wave-
length that is bundled through the lenses in the shuttle system and passed via the optic
fibers back to the color modules. In the color modules the light passes the beam divider
followed by two emission filters and is then transferred to the photomultiplier (CPM) for
detection.
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