Operation
30 Edition 03.19 qTOWER
3
/ qTOWER
3
G
7.3 Inserting samples / Starting the PCR analysis
WARNING! Biological hazard!
Take great care when working with potentially infectious material. Wear suitable protective
equipment, e.g. protective gloves.
WARNING
Risk of eye injury!
The rapid heating of the thermal block can cause liquids to boil explosively. Always wear
safety goggles during operation.
CAUTION! Hot surface!
The thermal block, the samples and the heated lid reach high temperatures. There is a risk
of burns during contact.
The qTOWER³ is equipped with a 96 well SBS sample block. This makes it suitable for
the use of 0.2 ml individual tubes, 8 well strips and 96 well micro titer plates. These and
other consumables can be ordered from Analytik Jena AG.
After pipetting the PCR samples into the wells these must be sealed with a transparent
optical adhesive film (sealing film). The optical transparency of the films affects the fluo-
rescence signal directly. Therefore, only use clear adhesive film as offered for real-time
PCR.
The strength of the lid contact pressure has been designed for a fully populated block. If
only a few samples are to be used in the block, place an additional two (empty) vessels
of the same height in the four corner positions of the block. Otherwise the sample ves-
sels or the heated lid may be damaged from excess contact pressure.
Fig. 9 Position of additional vessels for measurements with few samples
1. Prepare a real-time PCR project with complete details about the PCR run, fluores-
cence measurements and the sample layout of the PCR plate (see qPCRsoft man-
ual).
2. Open the lid. To do so, press in the red handle on the front until the lock clicks
open. Fold back the upper part of the device.
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