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Version 4.2 rev 06 Oct 2020

8.2 x 8.2 mm / 512 x 512 sensor

Lowest Noise Imaging EMCCD

Mechelle

4.11.1 eFFect oF aMbient teMpeRatuRe on tHe Relative eFFiciency coRRection

When the ambient temperature changes, the refractive index of the prisms will change which result in a shift of the

orders in the vertical direction, i.e. the light of a certain wavelength will fall on a different pixel on the sensor. Since there

is a small variation between the response of pixels, therefore the relative efciency correction will not be valid and the

corrected signal should look like that shown below:

Figure 26: Effect of the temperature change on relative efciency corrected signal

NOTE: If the ambient temperature is changed by ±3ºC, the Relative Efciency Correction will not be valid and the

corrected signal will look similar to the above, but the wavelength calibration will remain accurate.

4.11.2 Relative eFFiciency coRRection WitH sub-spectRuM

To carry out the relative efciency correction with sub-spectrum, the following procedure must be carried out:

1. Firstly, take a full spectrum as described previously.

2. Click the

button to select the wavelength range required and click update.

3. Close the wavelength selector window.

4. Click

and carry out the Relative Efciency Correction Procedure.

NOTES:

1. Do not take a scan between selecting the wavelength range and relative efciency procedure. If you do, the

button will not be active, i.e.

2. If the wavelength range is changed, the following message will be displayed:

The REC procedure must then be repeated. The software will also change the acquisition settings to Full Image for the

next acquisition only to prepare for Relative Efciency procedure. Just click the

button and carry out the procedure

again.

3. If the sub-spectrum set is between 400 - 975 nm, only a QTH Standard lamp is needed, so the deuterium

lamp is not necessary

Andor Technology Mechelle User Manual

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