Applied Biosystems 7300 Getting Started Guide

Appendix B Example RQ Experiment
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 87
Notes
B
8. Start the RQ run.
a. Select the Instrument tab. By default, the
Fast PCR conditions for the PCR step of the
two-step RT-PCR method are displayed for
the 7500 Fast instrument, whereas the
standard PCR conditions are displayed for
the 7300 and 7500 standard instruments (not
shown).
b. Select File > Save As, enter a name for the
RQ Plate document, then click Save.
c. Load the plate into the instrument.
d. Click Start.
After the run, a message indicates if the run is
successful or if errors were encountered.
9. Create an RQ Study document as described in
“Creating an RQ Study Document” on page 60.
a. Select File > New.
b. Select Relative Quantification (ddCt)
Study in the Assay drop-down list, then
click Next >.
IMPORTANT! RQ Study is an optional add-
on for the 7300 instrument; it is built-in for
the 7500 instrument and the 7500 Fast
instrument.
c. Click Add Plates to add plates to the study,
then click Open.
Note: You can add up to 10 RQ plates to an
RQ study.
d. Click Finish.

Applied Biosystems 7300 Specifications

General

Brand	Applied Biosystems
Model	7300
Category	Laboratory Equipment
Language	English