Applied Biosystems SimpliAmp Thermal Cycler User Manual

Predefined run methods
The following run methods (distributed across ten categories) are supplied with the
SimpliAmp
™
Thermal Cycler. A particular run method can appear in more than one
category. These run methods are based on our recommended protocols and should be
considered as starting points for the dierent applications. Your results may vary
between instrument models, so some optimization may be needed. Please note that
the ramp rates of these methods are set at 100%.
The following table lists the categories and the run methods they include.
Table 12 Pre-defined categories and run methods
Category
Run method
Blank Template
• General PCR Run Method
• Blank PCR 1
• Blank PCR 2
Basic PCR
• AmpliTaq
™
360 Method
• AmpliTaq
™
Method
• Basic PCR Taq Method
Hot Start
• AmpliTaq
™
360 Method
• AmpliTaq Gold
™
Method
• AmpliTaq Gold
™
Fast PCR Run Method
• Platinum
®
Taq DNA Polymerase Method
• Platinum
®
Taq SuperMix Method
Sequencing
• BigDye
™
Direct Cycle Sequence Method
• BigDye
™
Direct PCR Run Method
• BigDye
™
Kit Fast Run Method
• BigDye
™
Kit Standard Run Method
Optimization
• Time Release Run Method
• Touchdown PCR Run Method
RT-PCR SuperScript
®
III 1-Step System Method
E
SimpliAmp
™
Thermal Cycler User Guide 67

Applied Biosystems SimpliAmp Thermal Cycler Specifications

General

Brand	Applied Biosystems
Model	SimpliAmp Thermal Cycler
Category	Bicycle Accessories
Language	English