Applied Biosystems SimpliAmp Thermal Cycler - Page 68

Category Run method
High Fidelity
• AccuPrime
™
PFX 2-Step Method
• AccuPrime
™
PFX 3-Step Method
• AccuPrime
™
Taq High Fidelity Method
• Platinum
®
PFX 2-Step Method
• Platinum
®
PFX 3-Step Method
• Platinum
®
Taq High Fidelity Method
High Specificity
• AccuPrime
™
PFX 2-Step Method
• AccuPrime
™
PFX 3-Step Method
• AccuPrime
™
Taq DNA Polymerase Method
• AccuPrime
™
Taq High Fidelity Method
Next-Gen
Sequencing
TargetSeq
™
Exome Enrichment Method
Long PCR
• Elongase
®
2-Step Method
• Elongase
®
3-Step Method
• AccuPrime
™
Taq High Fidelity Method
• Platinum
®
Taq High Fidelity Method
Digital PCR (only for
Dual Flat block)
• 3D
• OpenArray
™
Genotyping
These run methods cannot be edited or deleted, but if you save them with a dierent
name or to a dierent folder, you can edit them.
The following sections provide the method details:
Note: For illustration purpose, the tables display maximum ramp rate. These will
however change with dierent block types.
AccuPrime
™
PFX 2-Step Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 1 35 1 — 50 105.0
Step 1 RampRate 6.0 6.0 6.0 —
Temperature 94.0 94.0 4.0
Hold Time 120 15 Infinite
Step 2 RampRate — 6.0 — —
Temperature 68.0
Hold Time 60
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
68 SimpliAmp
™
Thermal Cycler User Guide