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Applied Biosystems SimpliAmp Thermal Cycler - Editing Run Methods and Advanced Options

Applied Biosystems SimpliAmp Thermal Cycler
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3.
Irrespective of the source for creating a new run method, once you select one of
the methods, you will come to the Method Edit screen.
Figure 8 Preview of Run Method
See “Edit a run method“ on page 16 for instructions on editing a run method.
Note: You can choose to directly start a run without editing a run method. To
start a run, touch Next.
Edit a run method
The Edit Method screen serves as a preview of the run method you have selected.
If you do not want to edit any of the parameters, you can start a run directly by
touching Next.
1.
In the Edit Method screen for the run method that you have selected, touch Edit
or anywhere on the graphical temperature prole to start editing the parameters.
Figure 9 Options available in the Run Method preview screen
You will see that each of the numbers are surrounded by black elds indicating
that they are editable. At this point, you can edit any of the temperatures, times,
heated cover, number of cycles, and other parameters seen on the screen.
2.
Touch Manage Steps to manage the steps; for example, add or delete a stage or
step, or set more advanced parameters.
a.
Touch Add Steps to add a step.
Chapter 3
Create and run experiments on the instrument
Edit a run method
3
16
SimpliAmp
Thermal Cycler User Guide

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