Name: Applied Biosystems SimpliAmp Thermal Cycler
Brand: Applied Biosystems
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SuperScript

III 1-Step System Method

Cycle/Step

Stage Reaction

volume

(µL)

Cover

temp. (°C)

1 2 3 4

Cycles 1 40 1 — 50 105.0

Step 1 RampRate 6.0 6.0 6.0 —

Temperature 60.0 94.0 68.0

Hold Time 900 15 300

Step 2 RampRate 6.0 6.0 6.0 —

Temperature 94.0 55.0 4.0

Hold Time 120 30 Inﬁnite

Step 3 RampRate — 6.0 — —

Temperature 68.0

Hold Time 60

TargetSeq

™

Exome Enrichment Method

Cycle/Step

Stage Reaction

volume

(µL)

Cover

temp. (°C)

1 2 3 4

Cycles 72 – – – 50 105.0

Step 1 RampRate 6.0 – – –

Temperature 47.0

Hold Time 3600

Time Release Method

Cycle/Step

Stage Reaction

volume

(µL)

Cover

temp. (°C)

1 2 3 4

Cycles 1 40 1 — 50 105.0

Step 1 RampRate 6.0 6.0 6.0 —

Temperature 95.0 95.0 72.0

Hold Time 60 15 420

Step 2 RampRate — 6.0 6.0 —

Temperature 55.0 4.0

Hold Time 15 Inﬁnite

Appendix E Predeﬁned run methods

Location of power point and ports on the instrument

SimpliAmp

™

Thermal Cycler User Guide

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Applied Biosystems SimpliAmp Thermal Cycler Specifications

General

Brand	Applied Biosystems
Model	SimpliAmp Thermal Cycler
Category	Bicycle Accessories
Language	English

Applied Biosystems SimpliAmp Thermal Cycler User Manual

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Applied Biosystems SimpliAmp Thermal Cycler Specifications