Applied Biosystems SimpliAmp Thermal Cycler - Page 80

Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Step 3 RampRate 50 105.0— 6.0 — —
Temperature 72.0
Hold Time 30
Touchdown PCR Method
Cycle/Step
Stage Reaction
volume
(µL)
Cover
temp. (°C)
1 2 3 4
Cycles 20 10
— —
50 105.0
Step 1 RampRate 6.0 6.0
Temperature 94.0 94.0
Hold Time 15 15
Step 2 RampRate 6.0 6.0
Temperature 65.0 55.0
Hold Time 30 30
AutoDelta
Temperature
-0.5 —
AutoDelta
Starting
Cycle
1 —
3D
Cycle/Step
Stage Reaction
volume
(nL)
Cover
temp. (°C)
1 2 3 4
Cycles 1x 39x 1x
—
1 70.0
Step 1 RampRate 0.8 1.2 1.2
Temperature 96.0 60.0 60.0
Hold Time 600 120 120
Step 2 RampRate — 0.8 1.2
Temperature — 98.0 10.0
Hold Time — 30 ∞
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
80 SimpliAmp
™
Thermal Cycler User Guide