Name: Applied Biosystems SimpliAmp Thermal Cycler
Brand: Applied Biosystems
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OpenArray

™

Genotyping

Cycle/Step

Stage Reaction

volume

(nL)

Cover

temp. (°C)

1 2 3 4

Cycles 1x 50x

— —

33 70.0

Step 1 RampRate 1.3 1.3

Temperature 94.0 99.0

Hold Time 600 30

Step 2 RampRate — 1.1

Temperature — 54.0

Hold Time — 21

Step 3 RampRate — 0.1

Temperature — 57.0

Hold Time — 90

Appendix E Predeﬁned run methods

Location of power point and ports on the instrument

SimpliAmp

™

Thermal Cycler User Guide

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Applied Biosystems SimpliAmp Thermal Cycler Specifications

General