Applied Biosystems SimpliAmp Thermal Cycler - Page 81

OpenArray
™
Genotyping
Cycle/Step
Stage Reaction
volume
(nL)
Cover
temp. (°C)
1 2 3 4
Cycles 1x 50x
— —
33 70.0
Step 1 RampRate 1.3 1.3
Temperature 94.0 99.0
Hold Time 600 30
Step 2 RampRate — 1.1
Temperature — 54.0
Hold Time — 21
Step 3 RampRate — 0.1
Temperature — 57.0
Hold Time — 90
Appendix E Predefined run methods
Location of power point and ports on the instrument
E
SimpliAmp
™
Thermal Cycler User Guide 81