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Applied Biosystems SimpliAmp Thermal Cycler User Manual

Applied Biosystems SimpliAmp Thermal Cycler
92 pages
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For Research Use Only. Not for use in diagnostic procedures.
SimpliAmp
Thermal Cycler
USER GUIDE
Installation, Use, and Maintenance
for use with:
Invitrogen
and Applied Biosystems
PCR reagents
Catalog Number A24811
Publication Number MAN0009889
Revision C.0

Table of Contents

Questions and Answers:

Applied Biosystems SimpliAmp Thermal Cycler Specifications

General IconGeneral
BrandApplied Biosystems
ModelSimpliAmp Thermal Cycler
CategoryBicycle Accessories
LanguageEnglish

Summary

Product Information

Instrument Overview

Provides a general description of the SimpliAmp™ Thermal Cycler and its capabilities.

Instrument Operation and Setup

Touchscreen Menu Overview

Explains the layout and function of the main screen and its interactive icons.

Using the Touchscreen Interface

Details how to interact with the instrument's touchscreen and its common buttons for navigation and control.

Entering Text and Numerical Data

Guides users on inputting text and numerical data using the instrument's on-screen editors for various fields.

Experiment Management

Workflow and Method Creation

Outlines the general workflow for experiments and details on creating new run methods.

Editing Run Methods and Advanced Options

Instructions for editing run method parameters, including advanced options like VeriFlex, AutoDelta, and Ramp Rates.

Sample Loading and Run Execution

Covers the process of preparing and loading samples into the instrument and starting a thermal cycling run.

Monitoring Runs and Managing Data

Explains how to track run progress, monitor via screen, and export run reports.

Method and Folder Management

Details on organizing, copying, renaming, and deleting run methods and associated folders.

Instrument Maintenance and Updates

Routine Cleaning and Decontamination

Provides instructions for cleaning the instrument, touchscreen, and sample wells as part of routine maintenance.

Fuses, Firmware, and Self-Verification

Guides on replacing fuses, updating firmware, and performing instrument self-verification tests.

Installation and Connectivity

Package Contents and Initial Setup

Lists the items included in the package and the required materials for setting up the instrument.

System Installation and Network Configuration

Instructions for unpacking, setting up the instrument, and connecting it to a network via wired or wireless options.

User Management and Access Control

Details on creating user profiles, signing in, managing access modes, and sign-out timers.

Cloud and Mobile Integration

Guides on setting up cloud connectivity and using the mobile application for instrument control.

Recommended Instrument Settings

Covers configuration of instrument name, date/time, network connection, heated cover, and remote service.

Parts and Consumables

Instrument Part Numbers and Consumables

Provides a comprehensive list of consumables and parts with their corresponding catalog numbers for ordering.

Troubleshooting and Support

Common Troubleshooting Scenarios

Presents a table of common observations, possible causes, and recommended actions for resolving instrument issues.

Instrument Service and Returns

Outlines the procedure and requirements, including decontamination, for returning the instrument for repair.

Customer and Technical Support Information

Provides contact information and resources for obtaining technical assistance and product support.

Instrument Specifications

Site and Environmental Requirements

Specifies the necessary conditions and location requirements for installing the instrument to ensure proper operation.

Technical and System Specifications

Details the technical features, dimensions, power, performance specifications, and port layout of the instrument.

Predefined Run Methods

Method Categories and Descriptions

Lists the categories and specific pre-defined run methods available for various PCR applications.

Safety Information

Instrument Symbols and Hazard Warnings

Explains the meaning of symbols and hazard warnings found on the instrument and in the documentation.

General Instrument Safety Practices

Provides essential safety guidelines related to electrical hazards, physical injury, sharp edges, and proper usage.

Chemical and Biological Hazard Safety

Covers crucial precautions for handling chemicals and biological hazards, including storage, use, and waste disposal.

Documentation and Warranty

Related Documentation and Resources

Lists the documents shipped with the system and provides links to additional online resources and support.

Limited Product Warranty Information

Outlines the terms and conditions of the product warranty, including how to contact for support.