BTX ECM630 - Experiment-Specific Troubleshooting

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Publicaon 5423-020-REV 1.0 • www.btxonline.com
Arcing
Verify electrical component funconality. Verify properes of cell
sample. (Do cells need to be washed? Is the buer appropriate
for applicaon?) Verify properes of transfectant molecule (Is the
DNA well puried?) Try reducing the voltage or increase sample
volume unl arcing is no longer a problem.
Low (or no) Transfecon Eciency,
or Incorporaon
Verify physical, biological, chemical parameters. Verify delivery of
the pulse and pulse parameters. Is the voltage correct? Chamber gap?
Pulse length or appropriate instrument sengs? Number of pulses?
If so, follow Opmizaon Guidelines outlined.
Low Viability
Verify physical, biological, chemical parameters. Are the voltage,
chamber gap, pulse length (me constant), pulse number and
other instrument sengs correct? If so, reduce voltage, pulse
length, or number of pulses and reopmize protocol to improve
viability as outlined.
Voltage Drop
A drop in output voltage accompanies pulse delivery into highly
conducve samples (for example, PBS). Thus the displayed voltage
may in these situaons be less than that expected, given 5% full
scale accuracy and the monitoring accuracy of 5%.
Experiment Troubleshooting