BTX ECM630 - Applications; Bacteria and Yeast Transformation; Plant and Insect Transfection

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Publicaon 5423-020-REV 1.0 • www.btxonline.com
Bacteria and Yeast Transformaon
Electroporaon has long been recognized as the most ecient
means of transforming both gram-negave and gram-posive
bacteria and yeast. Gram-negave bacteria such as E. coli or
H. pylori are generally easier to transform than gram-posive
bacteria (e.g. S. pneumoniae) due to their cell wall composion.
Transformaon eciencies of 1x10
10
transformants/µg DNA
are commonly seen for gram negave bacteria, while for gram
posive bacteria, generally 1x10
6
transformants/µg DNA are
achievable.
Plant and Insect Transfecon
Electroporaon of plant ssue can be used to generate transgenic
crops that are useful in agricultural/horcultural applicaons.
Insect models are also widely used throughout the scienc
community to study development and gene regulaon and
funcon. The ability to introduce genes or molecules is essenal
to researchers working with either of these two species. This
is why researchers consistently turn to BTX for all of their
electroporaon needs.
Applications