BTX ECM630 - Product Overview

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Publicaon 5423-020-REV 1.0 • www.btxonline.com
Product Overview
High Throughput (HT) Electroporaon
The High Throughput (HT) 96- and 25-well systems oer mul-
well electroporaon technology for processing mulple samples
in seconds. Using the HT mul-well plates instead of tradional
cuvees, the researcher can transion from a single cuvee to
either a 96-well or 25-well electroporaon plate by using a plate
handler. This increases yields and the number of experiment runs
in a single day. Experiments take seconds to run, allowing for
quick and ecient opmizaon of the electrical and biological
parameters. Once opmized, samples are rapidly processed
increasing yields and saving valuable me and money.
The key to the HT System is the combinaon of the ECM 630
Generator with the Plate Handler and mul-well plates. The Plate
Handler holds the plates and delivers the pulse(s) to the wells
using parameters set in the ECM 630 generator. The HT 100 Plate
Handler provides manual track switching (45-0412, 45-0422).
Monitoring Opon
Addion of the Enhancer 3000 allows the researcher to monitor
and track key electrical parameters used in electroporaon
applicaons. The electrical pulse data is captured as both a
graphic display of the waveform and electrical output values
following each experiment.
The ECM 630 is an exponenal decay wave electroporaon
generator providing a broad range of voltage and me constants
for full exibility in varying transfecon applicaons. The ability to
select the resistance and capacitance values, and adjust the range
of voltages is the key to achieving the opmal me constants and
eld strengths needed for ecient transformaon of prokaryote
and eukaryote transfecon. The ECM 630 can perform the widest
range of electroporaon applicaons among commercially
available electroporaon generators.
Flexibility is important to a researcher, so BTX has designed the
ECM 630 to be a plug-and-play system that easily transions
between standard cuvees and 96-well electroporaon plates
using our high throughput plate handler.
Features
• Voltage range from 5 V to 3,000 V
• Time constant
LV 1 ms to 5.158 s
HV 0.5 ms to 133.875 ms
• Arc detecon
• Digital display of actual pulse data
Applicaons
• Transformaon of bacteria and yeast
• Transfecon of mammalian cells
• Transfecon of plant ssue and plant protoplasts
• High throughput 25- and 96-well electroporaon
CAUTION: FOR RESEARCH USE ONLY. NOT FOR
CLINICAL OR DIAGNOSTIC USE ON PATIENTS.