Setting Up the XP3000 for Your Application 4-4
2 Select syringe size.
Determine your volume and flowrate requirements. Select a syringe that
accommodates the smallest and largest volumes to be dispensed without refill, as
well as the desired flowrate (see Table 4-1). While smaller syringes allow better
accuracy and precision, a larger syringe allows more aliquots when multiple
aspirations or multiple dispenses are required, and they allow better breakoff and
longer seal life.
Table 4-1. Flowrate Ranges
Syringe Size Minimum Flowrate
(mL/min)
Maximum Flowrate
(mL/min)
50 µL
0.0025 2.6
100 µL
0.0050 5.23
250 µL
0.012 13.1
500 µL
0.025 26.2
1 mL 0.050 52.3
5 mL 0.25 261.6
3 Select tubing.
In tubing selection, the general rule is that smaller syringes work best with smaller
I.D. tubing and larger syringes with larger I.D. tubing. Most XP 3000 valve styles
have an internal I.D. of 0.059" (approx. 1/16"). For aspirate/dispense tubing a
thermal-drawn tip or tapered tip is most common, providing good breakoff and
excellent accuracy and precision for most applications. A necked-down tip may be
used when aspirating very small volumes of sample, i.e., 1 - 5
µL. A blunt-cut tip is
better suited for large volume applications. For tubing recommendations, see Table
4-2; for a description of the various types of tubing, see Appendix A, “Ordering
Information.”
Table 4-2. Tubing Recommendations
Syringe Size Aspirate/Dispense Tubing P/N Reagent Tubing P/N
50 µL, 100 µL, 250 µL
5133 721370
5723 *
500 µL, 1 mL, 2.5 mL
5133 4609
720595 5729
720597 721370
5 mL 4333 720592
720595 721370
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