Endress+Hauser analytikjena qTOWER iris - Page 32

Specifications qTOWER iris
32
Gradient function
Model with 96-capacity
sample block
Model with 384-capacity
sample block
Gradient 12 columns: 4to99°C, lin-
ear gradient tool
24 columns: 4to99°C, lin-
ear gradient tool
Max./min. gradient 40°C / 0.1°C 24°C / 0.1°C
qPCR application
Model with 96-capacity
sample block
Model with 384-capacity
sample block
Sensitivity 1nmol/l FAM at 30µl sam-
ple volume
1nmol/l FAM at 10µl sam-
ple volume
Measuring time approx. 6s for 96 wells for
a single measurement, inde-
pendent of the number of
colors (single-plate readout)
approx. 6s for 384 wells for
a single measurement, inde-
pendent of the number of
colors (single-plate readout)
Measurement range ±130000(±17bit)
Dynamic range 10 log stages
Fluorescence spectrometer
Model with 96-capacity
sample block
Model with 384-capacity
sample block
Measuring principle Fiber-optic shuttle system
with 8-fold scanner and
color modules for the excita-
tion and emission filters
Fiber-optic shuttle system
with 16-fold scanner and
color modules for the excita-
tion and emission filters
Light source 7 long-life, powerful LEDs (red, green, blue, white, UV)
Color modules ¡ 8 color and protein modules
¡ 6 positions in the device
Detector Highly sensitive PMT (photomultiplier tube)
Color modules
Description Excitation Emission Dyes (examples)
Color module 1
(blue)
455 ±15nm 515 ± 10nm FAM
TM
, SYBR
®
Green, ATTO425,
Cyan500
Color module 2
(green)
520 ± 10nm 560 ± 15nm JOE
TM
, HEX
TM
, VIC
®
, YakimaYel-
low
®
, TET
Color module 3 (yel-
low)
550 ± 15nm 585 ± 10nm TAMRA
TM
, ATTO550
Color module 4 (or-
ange)
580 ± 10nm 620 ± 15nm ROX
TM
, TexasRed
®
, Cy3.5
®
,
ATTO590
Color module 5
(red)
625 ± 10nm 670 ± 15nm Cy5
®
, ATTO633
Color module 6
(NIR1)
660 ± 10nm 710 ± 20nm Cy5.5
®
, ATTO665
Color module 7
(UV1)
375 ± 15nm 475 ± 15nm ATTO390, ATTO425
Color module Pro-
tein 1
465 ± 15nm 585 ± 15nm SYPRO
®
Orange