C–2
IDEXX VetTest* Chemistry Analyzer Operator’s Manual
Serum Sample Preparation
To prepare a serum sample:
Use the appropriate tube.1.
Use the appropriate sample collection device. 2.
Gently draw the patient’s blood and transfer it, if necessary, to a serum tube.3.
Notes:
If using a syringe and needle draw, be sure to remove the needle before t
transferring the blood into the tube to avoid hemolysis.
When using an evacuated tube, such as a Vacutainer* tube, allow the sample t
to draw naturally into the tube by vacuum.
Let the sample sit for a minimum of 20 to 30 minutes to ensure it is fully clotted.
(Clotting time may vary by sample.)
Centrifuge the sample for 10 minutes in a standard centrifuge (8,000 rpm) or for
TFDPOETJOBIJHITQFFE>12,000 rpm) centrifuge.
Using a transfer pipette, transfer the serum sample to a sample cup.
Process the sample immediately, or see the Sample StorageTFDUJPOPOQBHF$
for storage guidelines.
Plasma Sample Preparation
To prepare a plasma sample:
Use the appropriate tube. 1.
Note: Do not use EDTA. EDTA can cause interference with many analytes.
Use the appropriate sample collection device.2.
Gently draw the patient’s blood and transfer it, if necessary, to an appropriate 3.
plasma tube.
Notes:
If using a syringe and needle draw, be sure to remove the needle before t
transferring the blood into the tube to avoid hemolysis.
When using an evacuated tube, such as a Vacutainer* tube, allow the sample t
to draw naturally into the tube by vacuum.
Gently invert the sample for 30 seconds to mix it. Thoroughly mix the blood.
Note: Never shake the tube. This will cause hemolysis, which may interfere with
analysis.
Centrifuge the sample for 10 minutes in a standard centrifuge (8,000 rpm) or for
TFDPOETJOBIJHITQFFE>12,000 rpm) centrifuge.
Using a transfer pipette, transfer the plasma sample to a sample cup.
Process the sample immediately, or see the Sample StorageTFDUJPOPOQBHF$
for storage guidelines.
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