H–10
IDEXX VetTest* Chemistry Analyzer Operator’s Manual
Creatine kinase/CK
Creatine kinase is found at high activity only in the cytoplasm of cardiac and skeletal
muscle. This enzyme catalyzes the reversible phosphorylation of creatine by ATP to
creatine phosphate and ADP. Creatine phosphate is the major source of high energy
phosphate used in muscle contraction. (See EnzymesQBHF)
Principal reason for performing the test:
To identify lesions in skeletal or cardiac muscle.
Most common abnormality indicated by the test:
Skeletal muscle lesions attributable to trauma or vigorous exercise.
Special precautions in sample collection:
Samples must be processed and centrifuged immediately after drawing blood. Grossly
hemolyzed samples should not be used. Even slight hemolysis can cause marked
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lesion. It is important to determine that the patient has not been exercised vigorously
during the 12 hours prior to sampling. This may cause marked increases in creatine
kinase activity. EDTA and fluoride/oxalate will cause lowered CK results.
Limitations of procedure:
Elevated carbon dioxide levels may cause a significantly lowered result.
Complementary tests:
Creatine kinase determination provides a specific, sensitive indication of muscle cell
damage. Aspartate aminotransferase and lactate dehydrogenase activities may also
be measured but are less specific and show smaller corresponding increases when
muscle damage is present.
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