4.21 Connecting and rinsing the guard column
■■■■■■■■■■■■■■■■■■■■■■
34
■■■■■■■■
930 Compact IC Flex Oven SeS/PP/Deg (2.930.2560)
Use the syringe to aspirate eluent until there are no more air
bubbles in the eluent aspiration tubing.
5
Adjusting the contact pressure of the peristaltic pump
■ Adjust the contact pressure of the peristaltic pump (see "Setting
the contact pressure correctly", page 25).
6
Rinsing the instrument without columns
■ Rinse the instrument (without columns) with eluent for 10
minutes.
4.21 Connecting and rinsing the guard column
CAUTION
New guard columns are filled with a solution and sealed with stoppers
or caps on both sides.
Before inserting the guard column, ensure that this solution can be
mixed with the eluent being used (follow the information provided by
the manufacturer).
NOTE
The guard column may not be connected until after the instrument has
already been put into operation once (see Chapter 4.20, page 32). The
guard column and the separation column have to be replaced by a cou-
pling (6.2744.040) until then.
Accessories
For this step, you need the following accessories:
■ Guard column (suitable for separation column)