Molecular Devices SpectraMax M3 User Manual

SpectraMax M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
0112-0115 F 21
The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette
Reference and the Water Constant methods.
The actual pathlength, d, of a solvent is found from the following
equation:
When a Cuvette Reference is used for pathlength correction, the value
of k is obtained by taking optical density measurements on the fluid in
the cuvette at two wavelengths, 1000 and 900 nm:
When the Water Constant is used for pathlength correction, the value of
k is obtained from the instrument. This constant is saved in the
instrument in the factory and may differ slightly from instrument to
instrument.
Once the pathlength d is found, the following equation is used for the
pathlength correction:
PathCheck Pathlength Measurement Technology is applicable to almost
all biological/pharmaceutical molecules in aqueous solution because
they have little or no absorbance between 900 nm and 1000 nm at
concentrations normally used. PathCheck Pathlength Measurement
Technology can also be used with samples containing small amounts of
organics or high buffer concentrations by using the Cuvette Reference.
See
Water Constant or Cuvette Reference? on page 22.
dcm
Sample OD
1000
OD
900
–
k
-----------------------------------------------------------------=
k
Cuvette OD
1000
OD
900
–
=
OD
cm
---------
OD
Sample
dcm
------------------------=

Molecular Devices SpectraMax M3 Specifications

General

Brand	Molecular Devices
Model	SpectraMax M3
Category	Laboratory Equipment
Language	English