Molecular Devices SpectraMax M3 User Manual

Principles of Operation
30 0112-0115 F
The temperature feedback closed-loop control algorithms measure the
chamber air temperature, compare it to the temperature set point, and
use the difference to calculate the regulation of the heating cycles. This
technique results in accurate, precise control of the chamber
temperature with a temperature variation of the air inside the chamber
of less than 1.0°C. The temperature uniformity within the microplate
depends on its design and composition.
Read Types
The SpectraMax Multi-Mode Microplate Reader can perform four types
of read: endpoint, kinetic, spectrum and well scan. Instrument setup
parameters for each read type are discussed in the SoftMax
®
Pro
Software User Guide.
Endpoint Read
In an endpoint read, a reading of each microplate well is taken at a
single or multiple wavelengths.
Depending on the data mode selected in the Reduction window, values
can be reported as optical density or % Transmittance.
Kinetic Read
In a kinetic read the data are collected over time with multiple readings
taken at regular intervals. To achieve the shortest possible interval for
kinetic readings, choose wavelengths in ascending order.
Kinetic analysis can be performed for up to 99 hours. The kinetic read
interval depends upon the instrument setup parameters chosen in
SoftMax Pro Software.
Kinetic analysis has many advantages when determining the relative
activity of an enzyme in different types of microplate assays, including
ELISAs and the purification and characterization of enzymes and
enzyme conjugates. Kinetic analysis is capable of providing improved
dynamic range, precision, and sensitivity relative to endpoint analysis.
Spectrum Read
Spectral analysis measures optical density or % Transmittance across a
spectrum of wavelengths 200 nm to 1000 nm. For fluorescence or
luminescence mode, relative fluorescence units (RFU) or relative
luminescence units (RLU) values are reported.
All spectrum readings are made using the scanning monochromators of
the instrument.

Molecular Devices SpectraMax M3 Specifications

General

Brand	Molecular Devices
Model	SpectraMax M3
Category	Laboratory Equipment
Language	English