SpectraMax M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
0112-0115 F 45
Emission Cutoff Filter
The emission cutoff filters assist in reducing background. Sources of
background include stray excitation light and native fluorescence of
plate materials, sample constituents, and solvents (including water).
The default setting allows the instrument and SoftMax Pro Software to
determine which cutoff filter should be used (see Table for default
settings) in endpoint and kinetic modes. The spectral scan mode
default uses no cutoff filter.
Readings Per Well
The number of readings per well may vary between 1 (used for a quick
estimate) and 100 (for very precise measurements). The default
number of readings per well varies with the read mode: for
fluorescence, the default is 6, and for luminescence the display shows 1
read per well.
PMT Voltage
The voltage of the photomultiplier tube may be set to low (for higher
concentration samples), medium, or high (for lower concentration
samples) in all read modes. In endpoint and spectrum mode, there is
an additional setting, automatic, in which the instrument automatically
adjusts the PMT voltage for varying concentrations of sample in the
plate.
Temperature Control
The chamber of the SpectraMax Multi-Mode Microplate Reader is
isothermal at ambient as well as at elevated temperatures. The
temperature in the reading chamber may be adjusted from 2°C above
ambient to 60°C.
Note that assay optimization requires the use of a computer and
SoftMax Pro Software.
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