PicoQuant GmbH HydraHarp 400 Software V. 3.0.0.1
Table of Contents
1. Introduction...................................................................................................................................................... 5
2. Primer on Time–Correlated Single Photon Counting.......................................................................................6
2.1. Count Rates and Single Photon Statistics...............................................................................................7
2.2. Timing Resolution.................................................................................................................................... 8
2.3. Photon Counting Detectors......................................................................................................................9
2.3.1. Photomultiplier Tube (PMT)............................................................................................................. 9
2.3.2. Micro Channel Plate PMT (MCP).....................................................................................................9
2.3.3. Single Photon Avalanche Photo Diode (SPAD)...............................................................................9
2.3.4. Other and Novel Photon Detectors................................................................................................10
2.4. Principles Behind the TCSPC Electronics.............................................................................................10
2.5. Further Reading..................................................................................................................................... 14
3. Hardware and Software Installation...............................................................................................................15
3.1. General Installation Notes – Read This First.........................................................................................15
3.2. Software Installation............................................................................................................................... 16
3.3. Device Installation.................................................................................................................................. 17
3.4. Installation Troubleshooting...................................................................................................................18
3.5. Uninstalling the Software.......................................................................................................................18
4. Software Overview......................................................................................................................................... 19
4.1. Starting the Program.............................................................................................................................. 19
4.2. The Main Window..................................................................................................................................19
4.3. The Toolbar........................................................................................................................................... 21
4.4. The Control Panel.................................................................................................................................. 22
4.5. The Axis Panel....................................................................................................................................... 23
4.6. The Trace Mapping Dialog.....................................................................................................................24
4.7. Other Dialogs......................................................................................................................................... 24
5. Specific Measurement Tasks.........................................................................................................................25
5.1. Setting Up the Input Channels...............................................................................................................25
5.2. Setting Up and Running Interactive Measurements...............................................................................29
5.3. Time Tagged Mode Measurements.......................................................................................................30
5.3.1. System Requirements...................................................................................................................30
5.3.2. T2 Mode......................................................................................................................................... 30
5.3.3. T3 Mode......................................................................................................................................... 31
5.3.4. Running a basic TTTR Mode Measurement..................................................................................32
5.3.5. External Markers............................................................................................................................ 33
5.3.6. Using TTTR Mode Data Files........................................................................................................33
5.3.7. TTTR Mode Measurements with Real–Time Correlation...............................................................34
5.4. Time–Resolved Excitation and Emission Spectra..................................................................................36
6. Controls and Commands Reference..............................................................................................................41
6.1. Main Window......................................................................................................................................... 41
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