PicoQuant GmbH HydraHarp 400 Software V. 3.0.0.1
The approximate mono–exponential fluorescence lifetime can be obtained from a simple comparison of two
points in the curve with count rates in the ratio of 1 :
1
/
e
(e.g. 100 000 : 36 788). In this particular experiment
this resulted in a lifetime estimate of 2.55 ns in good agreement with published results for this dye. For a
precise measurement one would perform a numerical exponential fit with IRF deconvolution (typically
implemented as an iterative reconvolution). This would result in slightly shorter lifetimes since the IRF broadens
the decay. Indeed one can measure lifetimes significantly smaller than the IRF with this method. Additionally,
the rms residue from the fit can be used to assess the quality of the fit and thereby the reliability of the lifetime
measurement. The FluoFit decay fit software package from PicoQuant provides this functionality.
2.5. Further Reading
1. O’Connor, D.V.O., Phillips, D.:
Time–correlated Single Photon Counting
Academic Press, London, 1984
2. Lakowicz, J. R.:
Principles of Fluorescence Spectroscopy, 3rd Edition
Springer, New York, 2006
3. Kapusta, P., Wahl, M., Erdmann, R. (Eds.):
Advanced Photon Counting - Applications, Methods, Instrumentation
Springer Series on Fluorescence, Vol. 15, Springer, 2015
ISBN 978-3-319-15635-4
4. Ortmann U., Wahl M., Kapusta P.:
Time-resolved fluorescence: Novel technical solutions.
Springer Series on Fluorescence, Vol.5, p.259-275 (2008)
5. Koberling F., Kraemer B., Buschmann V., Ruettinger S., Kapusta P., Patting M., Wahl M., Erdmann R.:
Recent advances in photon coincidence measurements for photon antibunching and full correlation
analysis. Proceedings of SPIE, Vol.7185, 71850Q (2009)
6. Wahl M., Rahn H.-J., Röhlicke T., Kell G., Nettels D., Hillger F., Schuler B., Erdmann R.:
Scalable time-correlated photon counting system with multiple independent input channels.
Review of Scientific Instruments, Vol.79, 123113 (2008)
7. O’Connor, D.V.O., Ware, W.R., Andre, J.C.:
Deconvolution of fluorescence decay curves. A critical comparison of techniques.
J. Phys. Chem. 83, 1333–1343, 1979
8. Patting M., Wahl M., Kapusta P., Erdmann R.:
Dead-time effects in TCSPC data analysis.
Proceedings of SPIE, Vol.6583, 658307 (2007)
9. http://www.picoquant.com/scientific/references
Bibliography listing all publications with work based on PicoQuant instruments
10. http://www.picoquant.com/products/category/tcspc-and-time-tagging-modules/hydraharp-400-
multichannel-picosecond-event-timer-tcspc-module#papers
Bibliography listing all publications with work based on the HydraHarp 400
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