PicoQuant GmbH HydraHarp 400 Software V. 3.0.0.1
Trace Map
Launches the Trace Mapping dialog. The HydraHarp can measure histograms in up to 512 memory blocks. Out
of these, up to 8 curves can be displayed. The trace map dialog is used to select the curves to display. Tick the
individual "Show" boxes to display a curve. Select the number of the memory block you wish to map the
individual display curve to. Choose the active memory block you wish to use for the next measurement in the
HydraHarp control panel.
TTTR Mode
Opens the TTTR mode dialog box. Use this dialog to enter the acquisition time and the destination file for a
TTTR mode run. Make sure all other measurement parameters have been set and tested in interactive mode
before entering TTTR mode.
TTTR Real–Time Correlator
Opens the TTTR mode real–time correlator dialog box. Use this dialog for FCS preview during a TTTR mode
run. Make sure all other measurement parameters have been set and tested in interactive mode before
entering TTTR correlator mode. Changing to this mode takes a few seconds for reconfiguration of the
hardware. See also section 5.4.
Monochromator / TRES
In order to measure Time–Resolved Emission Spectra (TRES), the HydraHarp software provides a TRES
measurement mode, that allows to control a monochromator via stepper motors and automated collection of
spectrally resolved lifetime histograms. Clicking this button will launch the dialog for manual monochromator
control and TRES setup. There you can set up parameters such as the start, step and end of the wavelength
scan. There will be appropriate error messages if the monochromator / stepper and associated drivers are not
configured properly.
General Settings
Opens the software settings dialog box. Use this dialog to change standard settings of the HydraHarp software.
Notably these are: Display rate (0.1 to 1s), Draw mode (lines, stairs), Grid–checkbox, Prompt overwrite
(warning before overwriting existing data) and TTTR Marker Settings. The control connector of the HydraHarp
400 provides TTL inputs for synchronization signals. The markers can be enabled resp. disabled and recorded
at the rising or falling edge of the corresponding TTL signal. The active edges can be chosen here. The dialog
also allows selective disabling of warnings that you do not wish to receive. All settings will be kept in the
Windows registry and will be retrieved at the next program start. They will be individual to each user.
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