Defining the gradient 2-11
5. In the next row of the Time column, enter the run time at which you
want gradient event 2 to start.
6. In the Flow column, enter the solvent flow rate you want the pump to
reach by the start of gradient event 3.
7. In the %A or %B column, enter the percent solvent composition you
want the pump to reach by the start of gradient event 3.
8. In the Curve column, enter the curve profile for gradient event 2 to
follow while reaching the start of gradient event 3.
9. Repeat steps 5 through 8 for all gradient events your application
requires. You can define up to 50 gradient events.
10. If the system has 515 pumps as contributors and you want to know the
calculated 2545 BGM flow rate without 515 pumps, select Pump Only.
Result: The flow rate of the 2545 BGM pump appears in the Flow
column. This estimates the flow rate on the 2545 BGM’s front panel. The
%A and %B composition of the gradient are also calculated, based on the
contributions of the 515 pumps.
Example:
Entering the 515 flow rates is shown in Defining 515 pump
parameters on page 2-7.
• The flow rate for 515 (A) pump is 1.00 mL/min, and it does not
contribute to solvent flow.
• The flow rate for the 515 (B) pump, At-Column Dilution, is
1.25 mL/min, and it contributes to solvent flow B.
• The total flow rate for the System is 25.00 mL/min.
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