Operation
Axiostar plus Setting epi-fluorescence Carl Zeiss
B 40-815 e 12/01 3-15
The first epi-fluorescence setting is significantly
easier if you use the Plan-Neofluar 20x/0.50
objective and a strong fluorescent specimen.
Demonstration specimens can be used first.
• First leave the illumination beam closed;
move the filter stop carrier 2 (3-10/2) to the
closed position.
• Turn on the HBO 50 with the power supply
and let it warm up to operating
temperature for about 15 minutes.
• Use the adjustment handle (3-10/3) to
move the desired fluorescence filter
combination on the reflector carrier into the
beam path.
• Turn off the halogen lamp (on microscope)
and open illumination beam path. Move the
filter stop carrier 2 to the open position.
• Refocus on the specimen and, if necessary,
optimize the collector setting with the knob
(3-10/1) on the HBO 50. Set the collector so
that the illumination from the field of view
appears as equal as possible to the short-
wave excitation from the reflector module.
Correction of the collector position is no
longer necessary for modules with long-
wave excitation.
1 Knob for collector adjustment
2 Filter stop carrier 2
3 Adjustment handle
Fig. 3-10 Setting epi-fluorescence
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