Zeiss Axiostar plus - Measurement of Lengths

Operation
Carl Zeiss Measurement of lengths Axiostar plus
3-16 B 40-81 e 12/01
3.7 Measurement of lengths
Before starting:
The measurement of lengths using the Axiostar
plus requires the following, for example:
– stage micrometer, positive 5 + 100/100 y
D = 0.17 mm
– eyepiece crossline micrometer 10 : 100,
d = 26 mm
– An overview of available stage micrometers
and eyepiece reticles is provided in
chapter 1.6.
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The distance to be measured should be ≥ 5 mm
in the intermediate eyepiece image in order to
keep the influence of random measuring
deviations as low as possible.
Other measuring errors can occur if the
eyepiece has not been inserted into the tube
until stop.
Fig. 3-11 Length measurement
Settings:
Before the length measurement using the
microscope can be performed, the micrometer
or scale value of the used objective / eyepiece
reticle combination must be determined. This
scale value is exactly that distance in the
specimen which complies to one interval of the
used eyepiece crossline micrometer.
For calibration, align the scales of the stage
micrometer and the crossline micrometer
parallel to each other by turning the eyepiece,
and make the zero lines of both scales exactly
congruent.
If, for example, 99 increments (of 10 µm each)
of the stage micrometer correspond to exactly
100 increments of the crossline micrometer, as
in Fig. (3-11), the resulting scale value k’ for the
used objective / eyepiece reticle combination
(A-Plan 10x/0.25 and crossline micrometer
10:100) is
k‘ = x × 10 µm = 9.9 µm
After exchange of the stage micrometer for the
specimen to be measured, the measuring
distance of interest L results from the number
of increments of the eyepiece crossline
micrometer (tenth estimated) multiplied with
the scale value k’:
L = 35.5 × 9.9 µm = 351.5 µm.
Particularly large object structures can also be
determined by using the vernier scale
gradations (0.1 mm) on the mechanical stage.
Here, it might be necessary to determine the
distance to be measured through calculation
from a combined x and y measurement
(Pythagoras).
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