Agilent InfinityLab LC Series RID User Manual 17
1 Introduction to the Refractive Index Detector
How the Detector Operates
According to the formula below small angles of external deflection are
proportional to the difference between the refractive indices of medium 1 and
medium 2.
Where:
• γ = angle of external deflection
• n
2
= Refractive index of medium 2
• n
1
= Refractive index of medium 1
Factors that Affect Refractive Index
The refractive index of a medium is affected by a number of factors;
1 Wavelength
The refractive index varies with changes in the wavelength of the incident light
beam.
2 Density
As the density of the medium changes the refractive index changes. At a fixed
wavelength of incident light the changes in refractive index are generally linear
in relation to the changes in medium density.
The density of a medium will be affected by the following factors:
• Composition (if not a pure substance)
• Temperature
• Pressure