Agilent InfinityLab LC Series VWD User Manual 50
3Using the Module
Scanning with the VWD
Scanning with the VWD
1 Set up a run.
2 Start a run.
3 While running on the baseline, take a Blank Scan. A background scan is stored
in the memory.
4 When the peak of interest enters the flow cell, stop the flow (set flow rate to
zero or open the purge valve) and wait a few moments to stabilize the
concentration.
Access to the scan feature is only possible during run with stopped flow. The
spectrum is taken during a stop-flow condition only while the peak is kept in the
flow cell.
Table 6 Blank scan
• Step 1: Blank Scan: scan of the background (solvent) is stored in the
memory.
• Step 2: Sample Scan: scan of the peak of interest is taken while the
peak stays in the flow cell (stop-flow condition).
• Online Spectrum: Sample Scan minus Blank Scan.
Here the functions are inactive (grayed out). Will be active in run mode.
Turning off the pump would stop the run and no access to the sample scan is
possible.
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