Agilent InfinityLab LC Series VWD User Manual 71
5 Optimizing the Detector
Match the Flow Cell to the Column
Match the Flow Cell to the Column
The tables below recommend the flow cell that matches the column used. If
more than one selection is appropriate, use the larger flow cell to get the best
detection limit. Use the smaller flow cell for best peak resolution.
Standard HPLC Applications
Figure 19 Choosing a Flow Cell (Standard HPLC Applications)
Ultra fast separation with RRLC systems
Figure 20 Choosing a Flow Cell for G7114B (for ultra fast separation with RRLC systems)
• (+) For ultra fast analysis with step gradients the micro flow cell (2 µL, 3 mm)
gives the best performance
• (++) In high resolution analysis time is not the highest priority. Higher delay
volumes are accepted. Therefore we recommend to use the damper plus
mixer for a highest signal to noise.
• If longer columns (> 50 mm) for higher resolution are used, then the next
larger flow cell is the preferred choice for higher sensitivity.
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