108 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
5
Optimizing the Detector
Example: Optimization for Multiple Compounds
Example: Optimization for Multiple Compounds
Using PNAs as a sample, this example uses the described scanning functions.
Setting the Chromatographic Conditions
This example uses the following chromatographic conditions (the detector
settings are shown in Figure 37 on page 109).
Table 14 Chromatographic Conditions
Mobile phases A = water = 50 %
B = Acetonitrile = 50 %
Column Vydac-C18-PNA, 250 mm x 2.1 mm i.d. with 5 µm particles
Sample PAH 0.5 ng
Flow rate 0.4 ml/min
Compressibility A (water) 46
Compressibility B (Acetonitrile) 115
Stroke A and B auto
Time Table at 0 min % B=50
at 3 min % B=60
at 14.5 min % B=90
at 22.5 min % B=95
Stop time 26 min
Post time 8 min
Injection volume 1 µl
Oven temperature (1200) 30 °C
FLD PMT Gain PMT = 15
FLD Response time 4 s
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