182 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Verification and Calibration
Wavelength Verification and Calibration
The wavelength calibration is based on a Glycogen solution, which acts as a
strong elastic light scatterer (refer to ASTM Test Method E388-72-1993
“Spectral Bandwidth and Wavelength Accuracy of Fluorescence
Spectrometers”). The Glycogen solution is introduced into the flow cell and
then the built-in wavelength calibration functionality is used.
The algorithm is based on evaluating different grating orders and calculating
the wavelength scales of both, excitation and emission monochromator, by
applying the fundamental grating equation.
New instruments are shipped with a flow cell installed and have a factory wavelength
calibration.
If a flow cell is used other then the installed/shipped flow cell:
• verify that the installed flow cell is fixed very tight,
• perform a wavelength calibration using Glycogen (part of FLD Calibration Kit
(G7121-68001)).
A complete wavelength calibration is not always required. In most cases a quick
wavelength accuracy verification is sufficient enough, see Table 26 on page 183.
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