Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 187
Test Functions and Calibration
8
Wavelength Calibration Procedure
Wavelength Calibration Procedure
1 Preparation of the Glycogen Calibration Sample.
a To prepare 10 ml of the calibration solution you have to use 10 mg of the
Glycogen sample (a tolerance of ±20% is not critical).
b Fill the prepared amount into a suitable bottle/vial.
c Fill 10 ml of distilled water into the vial and shake.
d Wait 5 minutes and shake again. After 10 minutes the solution is ready.
When If application requires, or see Ta ble 2 7 on page 186.
Tools required Description
Laboratory balance
Parts required p/n Description
G7121-68001 FLD Calibration Kit
contains parts below
5063-6597 Calibration Sample, Glycogen
9301-1446 Syringe
9301-0407 Needle
5190-5111 Syringe filter, 0.45 µm, 100/pk
0100-1516 Fitting male PEEK, 2/pk
New instruments are shipped with a flow cell installed and have a factory wavelength
calibration.
If a flow cell is used other then the installed/shipped flow cell:
• verify that the installed flow cell is fixed very tight,
• perform a wavelength calibration using Glycogen (part of FLD Calibration Kit
(G7121-68001)).
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