188 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Calibration Procedure
2 Preparation of the Flow Cell.
a Flush the flow cell with water.
b Remove the inlet capillary from the flow cell.
c Take the syringe and fix the needle to the syringe adapter.
d Suck about 1.0 ml of the calibration sample into the syringe.
e Keep the syringe in a horizontal position.
f Remove the needle.
g Add the filter to the syringe and fit the needle to filter.
Figure 67 Syringe with Sample Filter
h Lift the needle tip and carefully eject approximately 0.5 ml to remove air
out of the syringe and to flush the needle.
i Add the PEEK fitting to the needle tip and fix both at the flow cell inlet.
j Slowly inject about 0.2 ml and wait for about 10 seconds to inject another
0.1 ml. This will assure that the cell is filled properly.
3 Wavelength Calibration.
a From the user interface start the FLD wavelength calibration.
• Agilent Lab Advisor: Calibrations
• Instant Pilot (G4208A): Maintenance > FLD > Calibration
Do not inject the calibration sample without the sample filter.
If the wavelength calibration process fails, refer to “Wavelength Calibration Failed” on
page 159.
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