184 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Calibration Process
Wavelength Calibration Process
Table 27 on page 186 shows the steps performed during the wavelength
calibration.
The excitation grating and the emission grating are calibrated using Rayleigh
stray light from the flow cell or cuvette measured with the photomultiplier
tube.
Prior to a wavelength calibration, a wavelength accuracy verification should be performed,
see “Wavelength Accuracy Test” on page 178. If the deviation is more than ±3 nm, the
wavelength calibration should be done as described in “Wavelength Calibration
Procedure” on page 187.
The duration of the wavelength calibration is about 20 minutes plus setup time for the
calibration sample and system. Depending on the maximum intensity found during this
scan, the PMT gain will be changed automatically and requires an additional 1 minute per
scan.
To Next Page
Loading...
Need help?
General
