Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 119
Optimizing the Detector
5
Optimization Overview
Optimization Overview
1 Setting the right PMT value
For most applications a setting of 10 is adequate (see “Finding the Best
Signal Amplification” on page 124). The FLD A/D converter exhibits a large
linear range making PMT switching unnecessary for most applications. For
example, if at high concentrations a peak is cut off; decrease the PMT
setting. Remember that low PMT settings decrease the signal to noise ratio.
The built-in PMT gain test uses the parameters in the detector. When using
the PMT gain test, the wavelength setting and lamp energy mode
(depending on Multiwavelength-Mode and Lamp-Economy) will affect the
PMT gain calculation.
2 Using an appropriate response time
For most applications a setting of 4 seconds is adequate (see “Selecting the
Best Response Time” on page 132). Only for high speed analyses (short
columns at high flow rates) a lower setting is recommended. Bear in mind
that even if the response time is too high fast peaks will appear a little
smaller and broader but retention time and peak areas are still correct and
reproducible.
Some features (e.g. spectrum acquisition, multi-wavelength detection) described in this
chapter are not available on the 1260 Infinity II Fluorescence Detector (G7121A).
PMT Gain Test
The PMT Gain test is not available in the Agilent OpenLAB CDS and G4208A Instant Pilot.
The PMT Gain test is available in the Agilent Lab Advisor since B.02.04 [093]. The PMT
Gain Test can be found under Instrument Control > Special Commands.
If you have changed one or more parameter(s), you have to press 'OK' to write down the
new settings into the FLD. Then re-enter 'FLD-Signals' and start the PMT gain test.
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