Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 181
Test Functions and Calibration
8
Wavelength Accuracy Test
Figure 65 Example of bad EX/EM maxima (no maximum found)
Interpretation of the Results
If the test fails, check for:
✔ correctly positioned flow cell,
✔ clean flow cell (flush with isopropanol and clean bi-distilled water),
✔ no air bubble(s) (check via fluorescence scan or visual check of
cell/cuvette),
✔ solvent inlet filter (may create air bubbles in flow cell).
✔ check optical path for contamination (service)
✔ check alignment of lamp / trigger pack assembly (service)
✔ perform a Wavelength Calibration
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