186 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
8
Test Functions and Calibration
Wavelength Calibration Process
If you encounter calibration problems:
1 Check for air bubbles in the flow cell.
2 Flush the flow cell with isopropanol.
3 Change the water.
Table 27 Wavelength Calibration Steps
Step Description Duration
1 Preparation max 30 s
2 Excitation rotation scan, full circle 60 s
3 Excitation rotation scan, high resolution 44 s
4 Excitation position scan, low resolution 55 s variable
5 Excitation position scan, high resolution 260 s variable
6.n Emission rotation scan, full circle (# of scans depends on the required PMT gain, 1
minute per scan)
61 s variable
6.n Em rotation scan, full circle" (instrument profile) 9 s
6.n Em rotation scan, full circle" (instrument profile) 9 s
6.n Em rotation scan, full circle" (instrument profile) 9 s
6.n Em rotation scan, full circle" (instrument profile) 9 s
7 Emission rotation scan, high resolution, part I 44 s
8 Emission rotation scan, high resolution, part II 44 s
9 Emission position scan, low resolution 50 s variable
10 Emission position scan, high resolution 250 s variable
Variable times means that they could be a little bit longer.
When the lamp is off, the calibration process will stop within the first two steps with "
Wavelength Calibration Failed" , see “Wavelength Calibration Failed” on page 159.
To Next Page
Loading...
Need help?
General
