126 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
5
Optimizing the Detector
Finding the Best Signal Amplification
Figure 47 PMT Gain Behavior
In this example the maximum output is around 220 LU and further increase of
the PMT (above 9) results in a signal overload (clipping) and drop of signal to
noise value.
1 Set the PMT-Gain Level
Now check with your highest concentration amount, that your highest peak
does not clip or overflow.
• If this check is ok, you finished the PMT-Gain-Level optimization.
Continue with "Set your Luminescence Units in LU".
• If the check shows that the highest concentration doesn't fit to the
selected range (e.g. by clipping), you may decrease the sensitivity of your
FLD by gradually decreasing the PMT-Level by 1 to get roughly half the
signal height by each step. Be aware that by that step you will lose
sensitivity at low signal levels (LOD).
To Next Page
Loading...
Need help?
General
