Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 23
Introduction to the Fluorescence Detector
1
Optical Unit
Figure 10 Dispersion of Light by a Grating
The grating is turned using a 3-phase brushless DC motor, the position of the
grating determining the wavelength or wavelength range of the light falling
onto the flow cell. The grating can be programmed to change its position and
therefore the wavelength during a run.
For spectra acquisition and multi-wavelength detection, the grating rotates at
4000 rpm.
The excitation and emission gratings are similar in design, but have different
blaze wavelengths. The excitation grating reflects most 1
st
order light in the
ultra-violet range around 250 nm, whereas the emission grating reflects better
in the visible range around 400 nm.
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