T720 Series Microscope
AmScope Proprietary Page 19 of 46
2.15 Using Darkfield Condenser Lenses (Optional Accessories)
Darkfield Illumination is a lighting method for increasing contrast. A darkfield
condenser lens produces bright images against a dark background for low contrast
biological specimens.
The dry darkfield condenser is for low power magnifications,
such as with the 4X, 10X or 40X objective lenses, with
numeric apertures of .65 or less.
The oil darkfield condenser is for high power magnifications,
the 40X and 100X objective lenses, with numeric apertures
of 1.25 or higher.
2.15.1 Installing a Darkfield Condenser Lens
1. Use the condenser lens adjustment knob to
lower the condenser so you have easy access to
the condenser knob.
2. Loosen the condenser lock screw and replace
the condenser lens.
3. Install the darkfield condenser lens and tighten
the condenser lock thumb screw on the
condenser holder.
When choosing a darkfield condenser, be sure the
condenserās numerical aperture is larger than the
numerical aperture of the objective lens.
If the objective lens aperture is larger than the aperture of
the darkfield condenser, direct light will be transmitted and
a darkfield will not be achieved.