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Applied Biosystems 7500 Getting Started Guide

Applied Biosystems 7500
106 pages
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Introduction and
Example
RQ Experiment
Designing an
RQ Experiment
Performing
Reverse
Transcription
Generating Data
from RQ Plates –
7300 or Standard
7500 System
Generating Data
from RQ Plates –
7500 Fast System
Analyzing Data in
an RQ Study
Primer Extended on mRNA
5
3
Reverse
Primer
5
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3
5
cDNA
STANDARD
STANDARD
STANDARD
FAST
FAST
FAST
Applied Biosystems 7300/7500/7500 Fast
Real-Time PCR System
Relative Quantification
Getting Started Guide

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Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

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