Applied Biosystems 7500 Getting Started Guide

Introduction and
Example
RQ Experiment
Designing an
RQ Experiment
Performing
Reverse
Transcription
Generating Data
from RQ Plates –
7300 or Standard
7500 System
Generating Data
from RQ Plates –
7500 Fast System
Analyzing Data in
an RQ Study
Primer Extended on mRNA
5′
3′
Reverse
Primer
5′
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
3′
5′
cDNA
STANDARD
STANDARD
STANDARD
FAST
FAST
FAST
Applied Biosystems 7300/7500/7500 Fast
Real-Time PCR System
Relative Quantification
Getting Started Guide

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)