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Applied Biosystems 7500 Getting Started Guide

Applied Biosystems 7500
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Chapter 2
Select the
PCR method
Specify the
components of
an RQ experiment
Select the
chemistry
Select one-step or
two-step RT-PCR
Choose probes
and primers
Chapter 3
Isolate total RNA
Adjust RNA
concentration
Convert
total RNA to cDNA
Primer Extended on mRNA
Reverse
Primer
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
cDNA
Create a new
RQ Study document
Configure
analysis settings
Adjust the
baseline and
threshold
Analyze and
view results
If necessary,
omit samples
Export AQ
Plate document,
if desired
Chapter 6
Chapter 5
Chapter 1
About the
7300/7500/7500 Fast
system
About Relative
Quantification
About
RQ Experiments
Prepare the
PCR Master Mix
Prepare the
reaction plate
Create a new
RQ Plate document
Program the
thermal cycling
conditions
Save the
RQ Plate
document
Start the run
View
RQ plate data
Chapter 4
(OR)
Designing
an RQ
Experiment
Performing
Reverse
Transcription
Primer Extended on mRNA
Reverse
Primer
Synthesis of 1st cDNA strand
cDNA
Oligo d(T) or random hexamer
cDNA
Performing
an RQ Study
Introduction
Generating
Data from
RQ Plates
Standard
Generating
Data from
RQ Plates
Fast
STANDARD
STANDARD
FAST
FAST
STANDARD
STANDARD
FAST
FAST
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide iii
Relative Quantification Experiment Workflow

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Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

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