EasyManuals Logo

Applied Biosystems 7500 Getting Started Guide

Applied Biosystems 7500
106 pages
To Next Page IconTo Next Page
To Next Page IconTo Next Page
To Previous Page IconTo Previous Page
To Previous Page IconTo Previous Page
Page #11 background imageLoading...
Page #11 background image
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 1
Notes
1
Chapter 1
Introduction
Introduction
About the
7300/7500/7500 Fast
system
About Relative
Quantification
About
RQ Experiments
D
es
i
gn
i
ng
an R
Q
E
xper
i
men
t
Performin
g
R
everse
Transcriptio
n
r
m
r
xt
n
n m
5
´
3
´
Revers
e
Prime
r
5
´
Sy
nthesis of 1st cDNA strand
cDNA
O
ligo d(T) or random hexame
r
3
´
5
´
cDNA
Performin
g
an R
Q
S
tud
y
OR
Generatin
g
Da
t
a
fr
om
RQ
Plate
s
S
tandard
STANDARD
TANDAR
G
eneratin
g
Da
t
a
fr
om
RQ
Plate
s
Fas
t
Seepage3
Seepage2
Seepage2

Table of Contents

Other manuals for Applied Biosystems 7500

Questions and Answers:

Question and Answer IconNeed help?

Do you have a question about the Applied Biosystems 7500 and is the answer not in the manual?

Applied Biosystems 7500 Specifications

General IconGeneral
Detection MethodFluorescence
Sample Capacity96-well plate
Thermal CyclerYes
Temperature Uniformity±0.5°C
TypeReal-Time PCR System
Thermal Accuracy±0.25°C
Detection Channels5
Weight45 kg
Power Requirements100-240 V, 50/60 Hz
Dynamic Range9 logs
Reaction Volume Range10-100 µL
Temperature Range4°C to 100°C
Thermal Cycler Block96-well
SoftwareSDS Software
DetectionMultiplex (up to 5 colors)

Related product manuals