Applied Biosystems 7500 Getting Started Guide

Chapter 4 Generating Data from RQ Plates – 7300 or Standard 7500 System
Preparing the Reaction Plate
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 23
Notes
STANDARD
STANDARD
STANDARD
4
Preparing the Reaction Plate
Standard vs.
Fast Plates
IMPORTANT! Make sure that you use the standard Optical 96-Well Plate on the 7500
Real-Time PCR system. Optical 96-Well Fast Plates will not fit into the standard block
correctly and will result in loss of data.
1. Label the reaction plates, ensuring that you include an endogenous control for each
sample type (for example, each tissue in a study comparing multiple tissues). If
samples are spread across multiple plates, each plate must have an endogenous
control. Additionally, every plate must include an endogenous control for every
sample type on the plate.
2. Into each well of the reaction plate, add 50 µL of the appropriate PCR master mix.
3. Keep the reaction plates on ice until you are ready to load them into the 7300/7500
system.
GR2386
7900
HS96 well plate comparison
100-µL
maximum
reaction
volume
Fast Plates Standard Plates
Notch at top-right
corner by A12
30-µL
maximum
reaction
volume
Notch at
top-left
corner
by A1
(PN 4346906) d(PN 4306737)

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)